ISG20 belongs to the the exonuclease superfamily. It is exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. It degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. It is involved in the antiviral function of IFN against RNA viruses.1) Horio, T., FEBS Lett. 577 (1-2), 111-116 (2004).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ISG20.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ISG20 antibody can be used for detection of ISG20 by ELISA at 1:1562500. ISG20 antibody can be used for detection of ISG20 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Daudi cells at 1.25 ug/ml.
Positive Control
1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
ISG20 antibody can be used for detection of ISG20 by ELISA at 1:1562500. ISG20 antibody can be used for detection of ISG20 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ISG20 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt