Application
Immunohistochemistry of JMJD1A in rat liver tissue with JMJD1A antibody at 5 μg/mL.Application
Immunofluorescence of JMJD1A in Rat Liver cells with JMJD1A antibody at 20 μg/mL.Application
Western blot analysis of JMJD1A in HepG2 cell lysate with JMJD1A antibody at 1 μg/mL.| Product Name | JMJD1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | JMJD1A Antibody: The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.1) Hoog C, Schalling M, Grunder-Brundell E, et al. Analysis of a murine male germ cell-specific transcript that encodes a putative zinc finger protein. Mol. Reprod. Dev.1991; 30:173-81. |
| Immunogen | JMJD1A antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50 - 100 of JMJD1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| JMJD1A antibody can be used for detection of JMJD1A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM3A |
|---|---|
| Alternative Names | JMJD1A Antibody: TSGA JMJD1 JHDM2A JHMD2A JMJD1A KIAA0742 TSGA Lysine-specific demethylase 3A JmjC domain-containing histone demethylation protein 2A |
| Molecular Weight(MW) | Predicted: 145 kDa Observed: 145 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (87%) |
| Tissue Specificity | This antibody will not cross-react with JMJD1B or JMJD1C. |
Application
Immunohistochemistry of JMJD1A in rat liver tissue with JMJD1A antibody at 5 μg/mL.
Application
Immunofluorescence of JMJD1A in Rat Liver cells with JMJD1A antibody at 20 μg/mL.
Application
Western blot analysis of JMJD1A in HepG2 cell lysate with JMJD1A antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | JMJD1A antibody can be used for detection of JMJD1A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | JMJD1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide. |
