Application
Immunohistochemistry of JMJD2A in rat liver tissue with JMJD2A antibody at 5 μg/mL.Application
Western blot analysis of JMJD2A in rat liver tissue lysate with JMJD2A antibody at (A) 1 and (B) 2 μg/mL.| Product Name | JMJD2A Antibody |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | JMJD2A Antibody: JMJD2, JHDM3A, JMJD2A, TDRD14A, JMJD2, KIAA0677, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A |
| Product description | JMJD2A Antibody: Members of the Jumonji domain 2 (JMJD2) family contain a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. The first member of this group, JMJD2A, is widely expressed in human tissues and cell lines and functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. JMJD2A can also form a complex with the androgen receptor (AR), a transcription factor that is pivotal for the development of prostate cancer. Overexpression of JMJD2A stimulates AR function and this stimulation is dependent on JMJD2A catalytic activity, suggesting that JMJD2A might be a critical protein with roles in cell proliferation and oncogenesis.1) Gray SG, Iglesias AH, Lizcano F, et al. Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein. J. Biol. Chem.2005; 31:28507-18. |
| Immunogen | JMJD2A antibody was raised against a 14 amino acid synthetic peptide from near the center of human JMJD2A. The immunogen is located within amino acids 340 - 390 of JMJD2A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| JMJD2A antibody can be used for detection of JMJD2A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM4A |
|---|---|
| Alternative Names | JMJD2A Antibody: JMJD2 JHDM3A JMJD2A TDRD14A JMJD2 KIAA0677 Lysine-specific demethylase 4A JmjC domain-containing histone demethylation protein 3A |
Application
Immunohistochemistry of JMJD2A in rat liver tissue with JMJD2A antibody at 5 μg/mL.
Application
Western blot analysis of JMJD2A in rat liver tissue lysate with JMJD2A antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
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| Application Notes | JMJD2A antibody can be used for detection of JMJD2A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | JMJD2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JMJD2A Antibody is supplied in PBS containing 0.02% sodium azide. |
