Application
Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 μg/mL.Application
Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 μg/mL.Application
Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | JMJD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JMJD4 Antibody: JmjC domain-containing protein 4, Jumonji domain-containing protein 4 |
| Product description | JMJD4 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the JMJD4 has not been identified. JMJD4 is expressed in multiple tissues and At least three isoforms of JMJD4 are known to exist.1) Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59. |
| Immunogen | JMJD4 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human JMJD4. The immunogen is located within amino acids 130 - 180 of JMJD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| JMJD4 antibody can be used for detection of JMJD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JMJD4 |
|---|---|
| Alternative Names | JMJD4 Antibody: JmjC domain-containing protein 4 Jumonji domain-containing protein 4 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (71%) |
| Tissue Specificity | This antibody will not recognize other members of the JMJD family. |
Application
Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 μg/mL.
Application
Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 μg/mL.
Application
Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | JMJD4 antibody can be used for detection of JMJD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | JMJD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JMJD4 Antibody is supplied in PBS containing 0.02% sodium azide. |
