Application
Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.| Product Name | JPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JPH1 Antibody: JP1, JP-1, JP1, Junctophilin-1, Junctophilin type 1 |
| Product description | JPH1 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).1) Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell.2000; 6:11-22. |
| Immunogen | JPH1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1. The immunogen is located within amino acids 470 - 520 of JPH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JPH1 |
|---|---|
| Alternative Names | JPH1 Antibody: JP1 JP-1 JP1 Junctophilin-1 Junctophilin type 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (87%) |
Application
Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
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| Application Notes | JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | JPH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | JPH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
