Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD (Ab-255). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using JunD (Ab-255).Application
Western blot analysis of lysed extracts from HT29 and 293 cells using JunD (Ab-255).| Product Name | JunD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP-1, MADH2, MADR2, AP-1 |
| Product description | Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.1) Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. |
| Immunogen | JunD (Ab-255) antibody was raised against a peptide sequence around aa. 253~257 (G-E-S-P-P) derived from Human JunD. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JUND |
|---|---|
| Alternative Names | AP-1 MADH2 MADR2 AP-1 |
| Molecular Weight(MW) | 38 kDa |
| Tissue Specificity | This antibody detects endogenous level of total JunD protein. |
| Entrez Gene | 3727 |
|---|---|
| Protein Accession | NP_005345.3 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD (Ab-255). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using JunD (Ab-255).
Application
Western blot analysis of lysed extracts from HT29 and 293 cells using JunD (Ab-255).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
