Application
Western blot analysis of K2C78 Antibody in Hela cell line lysates (35ug/lane)Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | K2C78 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Keratin, type II cytoskeletal 78, Cytokeratin-78, CK-78, Keratin-5b, Keratin-78, K78, Type-II keratin Kb40, KRT78, K5B, KB40 |
| Product description | This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified.1) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) |
| Immunogen | This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 amino acids from the Central region of human K2C78. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT78 |
|---|---|
| Alternative Names | Keratin type II cytoskeletal 78 Cytokeratin-78 CK-78 Keratin-5b Keratin-78 K78 Type-II keratin Kb40 KRT78 K5B KB40 |
| Molecular Weight(MW) | 57 kDa |
Application
Western blot analysis of K2C78 Antibody in Hela cell line lysates (35ug/lane)
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
