Application
Western blot analysis of KAT1 (HAT1) Antibody in HL60 cell line lysates (35ug/lane)| Product Name | KAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1 |
| Product description | Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.1) Gronroos, E., et al., Mol. Cell 10(3):483-493 (2002). |
| Immunogen | This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-58 amino acids from the N-terminal region of human KAT1 (HAT1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HAT1 |
|---|---|
| Alternative Names | Histone acetyltransferase type B catalytic subunit Histone acetyltransferase 1 HAT1 KAT1 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of KAT1 (HAT1) Antibody in HL60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
