Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: mouse cerebellum lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining KCTD11 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | KCTD11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Plays a role as a marker and a regulator of neuronal differentiation; Up-regulated by a variety of neurogenic signals, such as retinoic acid, epidermal growth factor/EGF and NGFB/nerve growth factor. Induces apoptosis, growth arrest and the expression of cyclin-dependent kinase inhibitor CDKN1B. Plays a role as a tumor repressor and inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Acts as an E3 ubiquitin-protein ligase towards HDAC1, leading to its proteasomal degradation. Functions as antagonist of the Hedgehog pathway on cell proliferation and differentiation by affecting the nuclear transfer of transcrition factor GLI1, thus maintaining cerebellar granule cells in undifferentiated state, this effect probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. When knock- down, Hedgehog antagonism is impaired and proliferation of granule cells is sustained. Activates the caspase cascade.1) Di Marcotullio L.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:10833-10838(2004). |
| Immunogen | This KCTD11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-53 amino acids from human KCTD11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000-1:2000 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCTD11 |
|---|---|
| Alternative Names | BTB/POZ domain-containing protein KCTD11 KCTD11 C17orf36 REN |
| Molecular Weight(MW) | 26 kDa |
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: mouse cerebellum lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining KCTD11 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
