Application
Western blot analysis of KDR (Y1175) Antibody in mouse lung tissue lysates (35ug/lane)Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KDR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KDR is a major growth factor for endothelial cells. This protein encodes one of the two receptors of the KDR. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc..1) Huang, D., et al. Cancer Res. 70(3):1053-1062(2010) |
| Immunogen | This KDR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1153-1182 amino acids from human KDR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDR |
|---|---|
| Alternative Names | Vascular endothelial growth factor receptor 2 VEGFR-2 Fetal liver kinase 1 FLK-1 Kinase insert domain receptor KDR Protein-tyrosine kinase receptor flk-1 CD309 KDR FLK1 VEGFR2 |
| Molecular Weight(MW) | 152 kDa |
Application
Western blot analysis of KDR (Y1175) Antibody in mouse lung tissue lysates (35ug/lane)
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
