Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (Ab-74). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Ab-74).Application
Western blot analysis of lysed extracts from HUVEC and 293 cells using Keratin 8 (Ab-74).| Product Name | Keratin 8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.1) Nakamichi I, et al. (2002) Mol Biol Cell; 13 (10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30 (15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91 (3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13 (11): 6742-6751. |
| Immunogen | Keratin 8 (Ab-74) antibody was raised against a peptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT8 |
|---|---|
| Alternative Names | K8 KO CK8 CK-8 CYK8 K2C8 CARD2 HSTF1 CK 8 Cytokeratin endo A K8 |
| Molecular Weight(MW) | 55 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Keratin 8 protein. |
| Entrez Gene | 3856 |
|---|---|
| Protein Accession | NP_002264.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (Ab-74). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Ab-74).
Application
Western blot analysis of lysed extracts from HUVEC and 293 cells using Keratin 8 (Ab-74).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
