Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | KIF2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KIF2A belongs to the kinesin-like protein family, MCAK/KIF2 subfamily. It contains 1 kinesin-motor domain. KIF2A plus end-directed microtubule-dependent motor required for normal brain development. It may regulate microtubule dynamics during axonal growth. KIF2A is implicated in formation of bipolar mitotic spindles. It has microtubule depolymerization activity. Hela cells lacking KIF2A show asymmetric or monopolar mitotic spindles. Osteosarcoma cells (U2OS) lacking KIF2A or KIF2B show disorganised or monopolar mitotic spindles.Kinesins, such as KIF2, are microtubule-associated motor proteins. For background information on kinesins, see MIM 148760.1) Jang, C.Y., (2008) J. Cell Biol. 181 (2), 255-267. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIF2A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| KIF2A antibody can be used for detection of KIF2A by ELISA at 1:62500. KIF2A antibody can be used for detection of KIF2A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIF2A |
|---|---|
| Alternative Names | KIF2A HK2 KIF2 CDCBM3 |
| Molecular Weight(MW) | 80 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | KIF2A antibody can be used for detection of KIF2A by ELISA at 1:62500. KIF2A antibody can be used for detection of KIF2A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KIF2A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
