The protein encoded by the KIF3B gene acts as a heterodimer with kinesin family member 3A to aid in chromosome movement during mitosis and meiosis. The encoded protein is a plus end-directed microtubule motor and can interact with the SMC3 subunit of the cohesin complex. In addition, the encoded protein may be involved in the intracellular movement of membranous organelles. This protein and kinesin family member 3A form the kinesin II subfamily of the kinesin superfamily.1) Nicole Weisschuh, et al., (2007) Exp Eye Res 85:450.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIF3B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
KIF3B antibody can be used for detection of KIF3B by ELISA at 1:312500. KIF3B antibody can be used for detection of KIF3B by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
KIF3B antibody can be used for detection of KIF3B by ELISA at 1:312500. KIF3B antibody can be used for detection of KIF3B by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KIF3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.