Application
Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 μg/mL.Application
Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 μg/mL.Application
Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | KIF5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KIF5 Antibody: The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the three isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.1) Hirokawa N. mRNA transport in dendrites: RNA granules, motors and tracks. J. Neurosci. 2006; 26:7139-42. |
| Immunogen | KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the center of human KIF5. The immunogen is located within amino acids 330 - 380 of KIF5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIF5A |
|---|---|
| Alternative Names | KIF5 Antibody: NKHC MY050 SPG10 D12S1889 NKHC1 Kinesin heavy chain isoform 5A Kinesin heavy chain neuron-specific 1 NKHC |
| Molecular Weight(MW) | Predicted: 105, 114 kDa Observed: 118 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | KIF5 has at least three known isoforms; this antibody recognizes all three. |
Application
Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 μg/mL.
Application
Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 μg/mL.
Application
Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KIF5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KIF5 Antibody is supplied in PBS containing 0.02% sodium azide. |
