Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. Kinesin consists of heavy and light chains both of which have been documented to bind a variety of potential linker or cargo proteins. KIF5B is an isoform of kinesin heavy chain.1) Diefenbach, R.J., et al., (2004) Biochem. Biophys. Res. Commun. 319 (3), 987-992.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIF5B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
KIF5B antibody can be used for detection of KIF5B by ELISA at 1:62500. KIF5B antibody can be used for detection of KIF5B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human brain at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Muscle.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
KIF5B antibody can be used for detection of KIF5B by ELISA at 1:62500. KIF5B antibody can be used for detection of KIF5B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KIF5B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.