Kin is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by the human ortholog.1) Despras, E., et al., (2003) Radiat.Res.159(6), 748-758.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIN.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
KIN antibody can be used for detection of KIN by ELISA at 1:2500. KIN antibody can be used for detection of KIN by western blot at 0.2-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney lysate.
Application
Antibody used in IHC on Human Spermatophore.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
KIN antibody can be used for detection of KIN by ELISA at 1:2500. KIN antibody can be used for detection of KIN by western blot at 0.2-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KIN antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.