Application
Immunohistochemistry of KIRREL2 in human pancreas tissue with KIRREL2 antibody at 5 μg/ml.Application
Immunofluorescence of KIRREL2 in human pancreas tissue with KIRREL2 antibody at 20 μg/ml.Application
Western blot analysis of KIRREL2 in 293 cell lysate with KIRREL2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | KIRREL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KIRREL2, also known as nephrin-like 3, is a type I transmembrane protein belonging to the immunoglobulin superfamily of cell adhesion molecules (1). KIRREL2 consists of five extracellular Ig-like repeats, a transmembrane domain, several glycosylation sites and a cytoplasmic domain that has a stretch of nine conserved residues (1,2). KIRREL2 has been found to be expressed in beta cells of pancreatic islets and is involved in the regulation of both glomerular and neural development (3,4). Downregulation of KIRREL2 is implicated in several proteinuric diseases (4,5).1) Sun C, Kilburn D, Lukashin A, et al. KIRREL2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets. Genomics 2003; 82:130-42. |
| Immunogen | KIRREL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KIRREL2. The immunogen is located within amino acids 550 - 600 of KIRREL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| KIRREL2 antibody can be used for detection of KIRREL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIRREL2 |
|---|---|
| Alternative Names | Kin of irregular chiasm-like protein 2 Kin of IRRE-like protein 2 NEPH3 Nephrin-like protein 3 NLG1 FILTRIN |
| Molecular Weight(MW) | Predicted: 64, 70, 78 kDa Observed: 70 kDa |
| Sequence Similarities | 2) Cat. No. 11-101 - Human Pancreas Tissue Slide |
| Tissue Specificity | KIRREL2 antibody is human, mouse and rat reactive. At least five isoforms are are known to exist. This antibody is predicted to not cross-react with other members of the KIRREL protein family. |
Application
Immunohistochemistry of KIRREL2 in human pancreas tissue with KIRREL2 antibody at 5 μg/ml.
Application
Immunofluorescence of KIRREL2 in human pancreas tissue with KIRREL2 antibody at 20 μg/ml.
Application
Western blot analysis of KIRREL2 in 293 cell lysate with KIRREL2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | KIRREL2 antibody can be used for detection of KIRREL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KIRREL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | KIRREL2 antibody is supplied in PBS containing 0.02% sodium azide. |
