Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use o for immunohistochemistry.Application
Western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).Application
Flow cytometric analysis of NCI-H460 cells usin(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | KIT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KIT is the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.1) Wardelmann, E., et al., Int. J. Cancer 106(6):887-895 (2003). Lennartsson, J., et al., Exp. Cell Res. 288(1):110-118 (2003). Sakuma, Y., et al., Cancer Sci 94(6):486-491 (2003). Araki, K., et al., Lung Cancer 40(2):173-180 (2003). Voytyuk, O., et al., J. Biol. Chem. 278(11):9159-9166 (2003). |
| Immunogen | This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human KIT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | KIT |
|---|---|
| Alternative Names | Mast/stem cell growth factor receptor Kit SCFR Piebald trait protein PBT Proto-oncogene c-Kit Tyrosine-protein kinase Kit p145 c-kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog CD117 KIT SCFR |
| Molecular Weight(MW) | 110 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use o for immunohistochemistry.
Application
Western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).
Application
Flow cytometric analysis of NCI-H460 cells usin(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
