KLF2/Lung Kruppel-like factor (LKLF) functions as a negative regulator of adipocyte differentiation. KLF2 is highly expressed in adipose tissue, and studies in cell lines and primary cells demonstrate that KLF2 is expressed in preadipocytes but not mature adipocytes.1) Basu, P., et al., (2005) Blood 106 (7), 2566-2571.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse KLF2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
KLF2 antibody can be used for detection of KLF2 by ELISA at 1:312500. KLF2 antibody can be used for detection of KLF2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse NIH-3T3 at 2.5 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
KLF2 antibody can be used for detection of KLF2 by ELISA at 1:312500. KLF2 antibody can be used for detection of KLF2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KLF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt