Application
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhaApplication
Western blot analysis in WiDr cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | KLF9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription.1) Du, H., et al. Biol. Reprod. 83(2):205-211(2010) |
| Immunogen | This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLF9 |
|---|---|
| Alternative Names | Krueppel-like factor 9 Basic transcription element-binding protein 1 BTE-binding protein 1 GC-box-binding protein 1 Transcription factor BTEB1 KLF9 BTEB BTEB1 |
| Molecular Weight(MW) | 27 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Pha
Application
Western blot analysis in WiDr cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
