Application
Western blot analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 μg/mL.Application
Immunohistochemistry of KLHL1 in rat brain tissue with KLHL1 antibody at 10 μg/mL.| Product Name | KLHL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | KLHL1 Antibody: The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.1) Nemes JP, Benzo KA, and Koob MD. The SCA8 transcript is an antisense RNA to a brain-specific transcript encoding a novel actin-binding protein (KLHL1). Human Mol. Gen. 2000; 9:1543-51. |
| Immunogen | KLHL1 antibody was raised against a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1. The immunogen is located within the first 50 amino acids of KLHL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHL1 |
|---|---|
| Alternative Names | KLHL1 Antibody: MRP2 KIAA1490 Kelch-like protein 1 |
| Molecular Weight(MW) | Predicted: 82 kDa Observed: 82 kDa |
| Sequence Similarities | 2) Cat. No. 1403 - Mouse Brain Tissue Lysate |
Application
Western blot analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 μg/mL.
Application
Immunohistochemistry of KLHL1 in rat brain tissue with KLHL1 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | KLHL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KLHL1 Antibody is supplied in PBS containing 0.02% sodium azide. |
