Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | KLHL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLHL3, FLJ40871, KIAA1129, MGC44594, PHA2D |
| Product description | KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.1) Schmutz, J., (2004) Nature 431 (7006), 268-274. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KLHL3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| KLHL3 antibody can be used for detection of KLHL3 by ELISA at 1:312500. KLHL3 antibody can be used for detection of KLHL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHL3 |
|---|---|
| Alternative Names | KLHL3 FLJ40871 KIAA1129 MGC44594 PHA2D |
| Molecular Weight(MW) | 65 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | KLHL3 antibody can be used for detection of KLHL3 by ELISA at 1:312500. KLHL3 antibody can be used for detection of KLHL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KLHL3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
