Application
Immunocytochemistry of KPNA5 in EL4 cells with KPNA5 antibody at 5 μg/mL.Application
Immunofluorescence of KPNA5 in EL4 cells with KPNA5 antibody at 20 μg/mL.Application
Western blot analysis of KPNA6 in EL4 cell lysate with KPNA5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | KPNA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA5 Antibody: SRP6, IPOA6, Importin subunit alpha-6, Karyopherin subunit alpha-5 |
| Product description | KPNA5 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein imort into the nucleus.1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene. Expr. 1997; 7:61-72. |
| Immunogen | KPNA5 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human KPNA5. The immunogen is located within amino acids 50 - 100 of KPNA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA5 |
|---|---|
| Alternative Names | KPNA5 Antibody: SRP6 IPOA6 Importin subunit alpha-6 Karyopherin subunit alpha-5 |
| Sequence Similarities | 2) Cat. No. 17-207 - EL4 Cell Slide |
| Tissue Specificity | KPNA5 antibody is predicted to not cross-react with other Importin alpha family members. |
Application
Immunocytochemistry of KPNA5 in EL4 cells with KPNA5 antibody at 5 μg/mL.
Application
Immunofluorescence of KPNA5 in EL4 cells with KPNA5 antibody at 20 μg/mL.
Application
Western blot analysis of KPNA6 in EL4 cell lysate with KPNA5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | KPNA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | KPNA5 Antibody is supplied in PBS containing 0.02% sodium azide. |
