Application
Antibody used in IHC on Human Skin at 1.25-2.5 ug/ml.Application
Antibody used in WB on Human 293T at 1 ug/ml.| Product Name | KRT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT1, CK1, EHK1, K1, KRT1A, EHK, EPPK, NEPPK |
| Product description | KRT1 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Schweizer, J., (2006) J. Cell Biol. 174 (2), 169-174. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| KRT1 antibody can be used for detection of KRT1 by ELISA at 1:62500. KRT1 antibody can be used for detection of KRT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT1 |
|---|---|
| Alternative Names | KRT1 CK1 EHK1 K1 KRT1A EHK EPPK NEPPK |
| Molecular Weight(MW) | 66 kDa |
Application
Antibody used in IHC on Human Skin at 1.25-2.5 ug/ml.
Application
Antibody used in WB on Human 293T at 1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | KRT1 antibody can be used for detection of KRT1 by ELISA at 1:62500. KRT1 antibody can be used for detection of KRT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KRT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
