Application
Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of KRT10 Antibody in 293 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | KRT10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP |
| Product description | KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in its gene are associated with epidermolytic hyperkeratosis.1) Morais,P., EurDermatol 19 (4), 333-336 (2009) |
| Immunogen | This KRT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human KRT10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT10 |
|---|---|
| Alternative Names | Keratin type I cytoskeletal 10 Cytokeratin-10 CK-10 Keratin-10 K10 KRT10 KPP |
| Molecular Weight(MW) | 59 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of KRT10 Antibody in 293 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
