Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
KRT12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT12 Antibdy for immunohistochemistry.Application
Western Blot at 1:2000 dilution + mouse eyeball lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:500 dilution + Y79 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goa| Product Name | KRT12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Keratin, type I cytoskeletal 12, Cytokeratin-12, CK-12, Keratin-12, K12, KRT12 |
| Product description | KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.1) Clausen, I., et al. Mol. Vis. 16, 954-960 (2010) : |
| Immunogen | This KRT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of human KRT12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT12 |
|---|---|
| Alternative Names | Keratin type I cytoskeletal 12 Cytokeratin-12 CK-12 Keratin-12 K12 KRT12 |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
KRT12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT12 Antibdy for immunohistochemistry.
Application
Western Blot at 1:2000 dilution + mouse eyeball lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:500 dilution + Y79 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goa| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
