Application
Flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KYNU Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of KYNU Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | KYNU Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kynureninase {ECO:0000255|HAMAP-Rule:MF_03017}, 3713 {ECO:0000255|HAMAP-Rule:MF_03017}, L-kynurenine hydrolase {ECO:0000255|HAMAP-Rule:MF_03017}, KYNU {ECO:0000255|HAMAP-Rule:MF_03017} |
| Product description | KYNU is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. KYNU is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway.1) Christensen,M., et.al., J. Inherit. Metab. Dis. 30 (2), 248-255 (2007) |
| Immunogen | This KYNU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-386 amino acids from the C-terminal region of human KYNU. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KYNU |
|---|---|
| Alternative Names | Kynureninase {ECO:0000255|HAMAP-Rule:MF_03017} 3713 {ECO:0000255|HAMAP-Rule:MF_03017} L-kynurenine hydrolase {ECO:0000255|HAMAP-Rule:MF_03017} KYNU {ECO:0000255|HAMAP-Rule:MF_03017} |
| Molecular Weight(MW) | 52 kDa |
Application
Flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KYNU Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of KYNU Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
