Application
Immunohistochemistry of LAMTOR1 in human small intestine tissue with LAMTOR1 antibody at 5 μg/mL.Application
Immunofluorescence of LAMTOR1 in human small intestine tissue with LAMTOR1 antibody at 20 μg/mL.Application
Western blot analysis of LAMTOR1 in A431 cell lysate with LAMTOR1 antibody at 1 μg/ml.| Product Name | LAMTOR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMTOR1 Antibody: p18, PDRO, C11orf59, p27RF-Rho, Ragulator1, PP7157, Ragulator complex protein LAMTOR1, Lipid raft adaptor protein p18 |
| Product description | LAMTOR1 is a 161 amino acid membrane protein belonging to the LAMTOR family. It has been identified to interact with and recruit the four Rag GTPases (Rag A-D) to the surface of lysosomes (1). The mTORC1 kinase complex is a critical component in the regulation of cell growth (2). As part of the ragulator complex, LAMTOR1 recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids (3). LAMTOR1 may be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes / lysosomes (4).1) Sancak Y, Bar-Peled L, Zoncu R, et al. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell 2010; 141:290-303. |
| Immunogen | Rabbit polyclonal LAMTOR1 antibody was raised against a 17 amino acid peptide near the amino terminus of human LAMTOR1 . The immunogen is located within the first 50 amino acids of LAMTOR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LAMTOR1 antibody can be used for detection of LAMTOR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LAMTOR1 |
|---|---|
| Alternative Names | LAMTOR1 Antibody: p18 PDRO C11orf59 p27RF-Rho Ragulator1 PP7157 Ragulator complex protein LAMTOR1 Lipid raft adaptor protein p18 |
| Molecular Weight(MW) | Predicted: 18 kDa Observed: 18 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | LAMTOR1 antibody is human, mouse and rat specific. LAMTOR1 antibody is predicted to not cross-react with other LAMTOR family proteins. |
Application
Immunohistochemistry of LAMTOR1 in human small intestine tissue with LAMTOR1 antibody at 5 μg/mL.
Application
Immunofluorescence of LAMTOR1 in human small intestine tissue with LAMTOR1 antibody at 20 μg/mL.
Application
Western blot analysis of LAMTOR1 in A431 cell lysate with LAMTOR1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | LAMTOR1 antibody can be used for detection of LAMTOR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LAMTOR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | LAMTOR1 antibody is supplied in PBS containing 0.02% sodium azide. |
