Application
Immunofluorescence of LASS5 in Human Brain cells with LASS5 antibody at 20 μg/mL.Application
Immunohistochemistry of LASS5 in human brain tissue with LASS5 antibody at 2.5 μg/mL.Application
Western blot analysis of LASS5 in rat brain tissue lysate with LASS5 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LASS5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LASS5 Antibody: Trh4, LASS5, Ceramide synthase 5, LAG1 longevity assurance homolog 5, CerS5 |
| Product description | LASS5 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.1) Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem.2003; 278:43452-9. |
| Immunogen | LASS5 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human LASS5. The immunogen is located within amino acids 90 - 140 of LASS5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LASS5 antibody can be used for detection of LASS5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LASS5 |
|---|---|
| Alternative Names | LASS5 Antibody: Trh4 LASS5 Ceramide synthase 5 LAG1 longevity assurance homolog 5 CerS5 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6. |
Application
Immunofluorescence of LASS5 in Human Brain cells with LASS5 antibody at 20 μg/mL.
Application
Immunohistochemistry of LASS5 in human brain tissue with LASS5 antibody at 2.5 μg/mL.
Application
Western blot analysis of LASS5 in rat brain tissue lysate with LASS5 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
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| Application Notes | LASS5 antibody can be used for detection of LASS5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LASS5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LASS5 Antibody is supplied in PBS containing 0.02% sodium azide. |
