Application
Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 μg/mL.Application
Western blot analysis of LASS6 in rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of LASS6 in Mouse Brain cells with LASS6 antibody at 5 μg/mL.| Product Name | LASS6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LASS6 Antibody: CERS5, LASS6, Ceramide synthase 6, LAG1 longevity assurance homolog 6, CerS6 |
| Product description | LASS6 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. Like the highly homologous LASS5, LASS6 is also an endoplasmic reticulum, multi-pass membrane protein. LASS6 is also involved in the synthesis of C14, C16 and C18-ceramide, but shows a preference for unsaturated fatty acids. LASS6 is broadly expressed in a wide range of tissues and microarray data suggests that it may play a role in cancer differentiation and early embryonic development.1) Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem.2003; 278:43452-9. |
| Immunogen | LASS6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of the human LASS6. The immunogen is located within the last 50 amino acids of LASS6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LASS6 antibody can be used for detection of LASS6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LASS6 |
|---|---|
| Alternative Names | LASS6 Antibody: CERS5 LASS6 Ceramide synthase 6 LAG1 longevity assurance homolog 6 CerS6 |
| Tissue Specificity | At least two isoforms of LASS6 are known to exist. This antibody is predicted not to cross-react with LASS5. |
Application
Immunohistochemistry of LASS6 in mouse brain tissue with LASS6 antibody at 2.5 μg/mL.
Application
Western blot analysis of LASS6 in rat brain tissue lysate with LASS6 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of LASS6 in Mouse Brain cells with LASS6 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | LASS6 antibody can be used for detection of LASS6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LASS6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LASS6 Antibody is supplied in PBS containing 0.02% sodium azide. |
