LDB2 belongs to the LDB family. LDB2 binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LIM domains are required for both inhibitory effects on LIM homeodomain transcription factors and synergistic transcriptional activation events. The inhibitory actions of the LIM domain can often be overcome by the LIM co-regulators known as CLIM2, LDB2 and NLI. LIM homeoproteins and CLIMs are involved in a variety of developmental processes.1) Colland, F., (2004) Genome Res. 14 (7), 1324-1332 .
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LDB2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
LDB2 antibody can be used for detection of LDB2 by ELISA at 1:312500. LDB2 antibody can be used for detection of LDB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate
Application Notes
LDB2 antibody can be used for detection of LDB2 by ELISA at 1:312500. LDB2 antibody can be used for detection of LDB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store LDB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.