Application
LDB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | LDB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIM domain-binding protein 2, LDB-2, Carboxyl-terminal LIM domain-binding protein 1, CLIM-1, LIM domain-binding factor CLIM1, LDB2, CLIM1 |
| Product description | Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This LDB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the C-terminal region of human LDB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDB2 |
|---|---|
| Alternative Names | LIM domain-binding protein 2 LDB-2 Carboxyl-terminal LIM domain-binding protein 1 CLIM-1 LIM domain-binding factor CLIM1 LDB2 CLIM1 |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
LDB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
