Application
LDHA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of LDHA Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | LDHA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA |
| Product description | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDHA |
|---|---|
| Alternative Names | L-lactate dehydrogenase A chain LDH-A Cell proliferation-inducing gene 19 protein LDH muscle subunit LDH-M Renal carcinoma antigen NY-REN-59 LDHA |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
LDHA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of LDHA Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
