Application
Confocal immunofluorescent analysis of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of hLep-R149 in MDA-MB231 cell line lysates (35ug/lane)| Product Name | Leptin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leptin, Obese protein, Obesity factor, LEP, OB, OBS |
| Product description | Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.1) Tank, J., et al., J. Clin. Endocrinol. Metab. 88(10):4955-4959 (2003). |
| Immunogen | This Leptin (LEP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the C-terminal region of human Leptin (LEP). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LEP |
|---|---|
| Alternative Names | Leptin Obese protein Obesity factor LEP OB OBS |
| Molecular Weight(MW) | 19 kDa |
Application
Confocal immunofluorescent analysis of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of hLep-R149 in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
