Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using LIMK1 (Phospho-Thr508).Application
Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508).| Product Name | LIMK1 (phospho Thr508) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.1) Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275 (5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1 (5): 253-9. |
| Immunogen | LIMK1 (Phospho-Thr508) antibody was raised against a peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1. |
| Modification | p-Thr508 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIMK1 |
|---|---|
| Alternative Names | LIMK LIMK-1 C1orf215 LAP18 OP18 kinase LIMK1 |
| Molecular Weight(MW) | 72 kDa |
| Tissue Specificity | This antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508. |
| Entrez Gene | 3984 |
|---|---|
| Protein Accession | NP_002305.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using LIMK1 (Phospho-Thr508).
Application
Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
