Application
Immunohistochemistry of lipe in human lymph node tissue with lipe antibody at 2.5 μg/mL.Application
Immunofluorescence of Lipe in Human Lymph Node cells with Lipe antibody at 20 μg/mL.Application
Western blot analysis of Lipe in human lymph node tissue lysate with Lipe antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | Lipe Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lipe Antibody: HSL, LHS, Hormone-sensitive lipase, HSL |
| Product description | Lipe Antibody: Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.1) Yeaman SJ. Hormone-sensitive lipase - new roles for an old enzyme. Biochem. J. 2004; 379:11-22. |
| Immunogen | Lipe antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Lipe. The immunogen is located within amino acids 920 - 970 of Lipe. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIPE |
|---|---|
| Alternative Names | Lipe Antibody: HSL LHS Hormone-sensitive lipase HSL |
| Molecular Weight(MW) | Predicted: 85, 90, 110, 118 kDa Observed: 90 kDa |
| Sequence Similarities | 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
| Tissue Specificity | Multiple isoforms of Lipe are known to exist. |
Application
Immunohistochemistry of lipe in human lymph node tissue with lipe antibody at 2.5 μg/mL.
Application
Immunofluorescence of Lipe in Human Lymph Node cells with Lipe antibody at 20 μg/mL.
Application
Western blot analysis of Lipe in human lymph node tissue lysate with Lipe antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1369 - Human Lymph node Tissue Lysate |
|---|---|
| Application Notes | Lipe antibody can be used for detection of Lipe by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Lipe antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Lipe Antibody is supplied in PBS containing 0.02% sodium azide. |
