Application
Western blot analysis of LKB1 in PC-3 cell lysate with LKB1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.| Product Name | LKB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LKB1 Antibody: PJS, LKB1, hLKB1, PJS, Liver kinase B1 |
| Product description | LKB1 Antibody: The LKB1 serine/threonine protein kinase was initially identified as a tumor suppressor gene mutated in human Peutz-Jeghers syndrome (PJS), a condition resulting in the growth of numerous intestinal polyps classed as hamartomas. LKB1 exists as a heterotrimeric complex with two other proteins, Ste20-related adaptor protein (STRAD) and MO25. Together, this complex can phsophorylate and activate the AMP-activate protein kinase (AMPK). Following AMPK activation by LKB1, AMPK then phosphorylates TSC1 and TSC2, key components of the metabolism-regulating TOR signaling pathway, which antagonizes the activation for the TOR pathway. LKB1 has also been shown to play a fundamental role in controlling the spatial orientation of structures required to maintain an ordered, polarized epithelium.1) Hemminki A, Markie D, Tomlinson I, et al. A serine/threonine kinase gene defective in Peutz-Jeghers syndrome. Nature 1998; 391:184-7. |
| Immunogen | LKB1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human LKB1. The immunogen is located within amino acids 310 - 360 of LKB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| LKB1 antibody can be used for detection of LKB1 by Western blot at 1 μg/mL. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK11 |
|---|---|
| Alternative Names | LKB1 Antibody: PJS LKB1 hLKB1 PJS Liver kinase B1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (100%) |
Application
Western blot analysis of LKB1 in PC-3 cell lysate with LKB1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | LKB1 antibody can be used for detection of LKB1 by Western blot at 1 μg/mL. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LKB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LKB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
