Application
Immunohistochemistry of LMX1A in human brain tissue with LMX1A antibody at 2.5 μg/mL.Application
Immunofluorescence of LMX1A in mouse brain tissue with LMX1A Antibodyat 20 μg/mL.Application
Immunofluorescence of LMX1A in human brain tissue with LMX1A antibody at 20 μg/mL.Application
Immunohistochemistry of LMX1A in mouse brain tissue with LMX1A Antibodyat 5 μg/mL.Application
Western blot analysis of LMX1A in rat brain tissue lysate with LMX1A antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LMX1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LMX1A Antibody: LMX1, LMX1.1, LIM homeobox transcription factor 1-alpha |
| Product description | LMX1A Antibody: The LIM homeobox transcription factor 1 (LMX1A) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A binds an A/T-rich sequence and functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. While LMX1A is crucial for the differentiation of dopamine neurons, the related LMX1B appears to be important for both the development and the survival of these cells. It has been suggested that LMX1A might be a useful biomarker or risk factor in tumor aggressiveness.1) Failli V, Bachy I and Retaux S. Expression of the LIM-homeodomain gene Lmx1a (dreher) during development of the mouse nervous system. Mech. Dev. 2002; 118:225-8. |
| Immunogen | Rabbit polyclonal LMX1A antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A. The immunogen is located within amino acids 300 - 350 of LMX1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LMX1A antibody can be used for detection of LMX1A by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LMX1A |
|---|---|
| Alternative Names | LMX1A Antibody: LMX1 LMX1.1 LIM homeobox transcription factor 1-alpha |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B. |
Application
Immunohistochemistry of LMX1A in human brain tissue with LMX1A antibody at 2.5 μg/mL.
Application
Immunofluorescence of LMX1A in mouse brain tissue with LMX1A Antibodyat 20 μg/mL.
Application
Immunofluorescence of LMX1A in human brain tissue with LMX1A antibody at 20 μg/mL.
Application
Immunohistochemistry of LMX1A in mouse brain tissue with LMX1A Antibodyat 5 μg/mL.
Application
Western blot analysis of LMX1A in rat brain tissue lysate with LMX1A antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | LMX1A antibody can be used for detection of LMX1A by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LMX1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LMX1A Antibody is supplied in PBS containing 0.02% sodium azide. |
