Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Human 721_B at 1 ug/ml.Application
Antibody used in WB on Human 721_B at 1 ug/ml.Application
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.| Product Name | LMX1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LMX1A, LMX-1, LMX1, LMX1.1, MGC87616 |
| Product description | Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin. Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-199 AL160058.8 14249-14447 c 200-2447 AK127724.1 362-2609 2448-3382 AL390730.12 9415-10349 c1) Gregory, S.G., (2006) Nature 441 (7091), 315-321. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LMX1A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| LMX1A antibody can be used for detection of LMX1A by ELISA at 1:62500. LMX1A antibody can be used for detection of LMX1A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LMX1A |
|---|---|
| Alternative Names | LMX1A LMX-1 LMX1 LMX1.1 MGC87616 |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Application
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | LMX1A antibody can be used for detection of LMX1A by ELISA at 1:62500. LMX1A antibody can be used for detection of LMX1A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store LMX1A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
