Application
Immunohistochemistry of LMX1B in human brain tissue with LMX1B antibody at 5 μg/mL.Application
Immunofluorescence of LMX1B in mouse brain tissue with LMX1B Antibodyat 20 μg/mL.Application
Immunohistochemistry of LMX1B in mouse brain tissue with LMX1B Antibodyat 5 μg/mL.Application
Immunofluorescence of LMX1B in human brain tissue with LMX1B antibody at 20 μg/mL.Application
Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LMX1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LMX1B Antibody: NPS1, LMX1.2, LIM homeobox transcription factor 1-beta |
| Product description | LMX1B Antibody: The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning; LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.1) Hobert O and Westphal H. Functions of LIM-homeobox genes. Trends Genet. 2000; 16:75-83. |
| Immunogen | Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B. The immunogen is located within amino acids 280 - 330 of LMX1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LMX1B |
|---|---|
| Alternative Names | LMX1B Antibody: NPS1 LMX1.2 LIM homeobox transcription factor 1-beta |
| Molecular Weight(MW) | 45 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A. |
| Entrez Gene | 4010 |
|---|---|
| Protein Accession | NP_001167617 |
Application
Immunohistochemistry of LMX1B in human brain tissue with LMX1B antibody at 5 μg/mL.
Application
Immunofluorescence of LMX1B in mouse brain tissue with LMX1B Antibodyat 20 μg/mL.
Application
Immunohistochemistry of LMX1B in mouse brain tissue with LMX1B Antibodyat 5 μg/mL.
Application
Immunofluorescence of LMX1B in human brain tissue with LMX1B antibody at 20 μg/mL.
Application
Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LMX1B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LMX1B Antibody is supplied in PBS containing 0.02% sodium azide. |
