Application
Immunohistochemistry of LPIN1 in human small intestine tissue with LPIN1 antibody at 5 μg/ml.Application
Immunofluorescence of LPIN1 in human small intestine tissue with LPIN1 antibody at 20 μg/ml.Application
Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.| Product Name | LPIN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LPIN1 Antibody: PAP1, KIAA0188, Phosphatidate phosphatase LPIN1, Lipin-1 |
| Product description | LPIN1 Antibody: LPIN1, also known as PAP1, is a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol (reviewed in 1). LPIN1 is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism (1,2). Mutations in LPIN1 are associated with metabolic diseases such as type 2 diabetes and recurrent acute myoglobinuria (3,4) and it is also a candidate for several human lipodystrophy syndromes (5).1) Reue K and Zhang P. The lipin protein family: dual roles in lipid biosynthesis and gene expression. FEBS Lett. 2008; 582:90-6. |
| Immunogen | LPIN1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human LPIN1. The immunogen is located within the last 50 amino acids of LPIN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LPIN1 antibody can be used for detection of LPIN1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LPIN1 |
|---|---|
| Alternative Names | LPIN1 Antibody: PAP1 KIAA0188 Phosphatidate phosphatase LPIN1 Lipin-1 |
| Molecular Weight(MW) | Predicted: 107 kDa Observed: 125 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | LPIN1 antibody is human and mouse reactive. At least four isoforms of LPIN1 are known to exist. |
| Entrez Gene | 23175 |
|---|---|
| Protein Accession | NP_001248357 |
Application
Immunohistochemistry of LPIN1 in human small intestine tissue with LPIN1 antibody at 5 μg/ml.
Application
Immunofluorescence of LPIN1 in human small intestine tissue with LPIN1 antibody at 20 μg/ml.
Application
Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | LPIN1 antibody can be used for detection of LPIN1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LPIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | LPIN Antibody is supplied in PBS containing 0.02% sodium azide. |
