Application
Immunohistochemistry of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 5 μg/mL.Application
Immunofluorescence of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 20 μg/mL.Application
Western blot analysis of LRRFIP2 in rat colon tissue lysate with LRRFIP2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | LRRFIP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LRRFIP2 Antibody: HUFI-2, Leucine-rich repeat flightless-interacting protein 2, LRR FLII-interacting protein 2 |
| Product description | LRRFIP2 Antibody: The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of beta-catenin. LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-κB during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.1) Fong KS and de Couet HG. Novel proteins interacting with the leucine-rich repeat domain of human flightless-I identified by the yeast two-hybrid system. Genomics 1999; 58:146-57. |
| Immunogen | LRRFIP2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LRRFIP2. The immunogen is located within amino acids 620 - 670 of LRRFIP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LRRFIP2 antibody can be used for detection of LRRFIP2 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRRFIP2 |
|---|---|
| Alternative Names | LRRFIP2 Antibody: HUFI-2 Leucine-rich repeat flightless-interacting protein 2 LRR FLII-interacting protein 2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | Three isoforms of LRRFIP2 are known to exist; this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members. |
Application
Immunohistochemistry of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 5 μg/mL.
Application
Immunofluorescence of LRRFIP2 in human colon tissue with LRRFIP2 antibody at 20 μg/mL.
Application
Western blot analysis of LRRFIP2 in rat colon tissue lysate with LRRFIP2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1472 - Rat Colon Tissue Lysate |
|---|---|
| Application Notes | LRRFIP2 antibody can be used for detection of LRRFIP2 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LRRFIP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LRRFIP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
