Application
Immunohistochemistry of LSD1 in human spleen tissue with LSD1 antibody at 5 μg/mL.Application
Western blot analysis of LSD1 in A549 cell lysate with LSD1 antibody at 1 μg/ml.| Product Name | LSD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lysine-specific histone demethylase, amine oxidase flavin containing domain protein 2, AOF2 |
| Product description | Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.1) Shi Y, Lan F, Matson C, et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119:941-53. |
| Immunogen | LSD1 antibody was raised against a 19 amino acid peptide near the amino terminus of human LSD1. The immunogen is located within amino acids 90 - 140 of LSD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| LSD1 antibody can be used for detection of LSD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM1A |
|---|---|
| Alternative Names | Lysine-specific histone demethylase amine oxidase flavin containing domain protein 2 AOF2 |
| Molecular Weight(MW) | Predicted: 93, 96 kDa Observed: 93, 96 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | LSD1 antibody is human, mouse, and rat reactive. At least two isoforms of LSD1 are known to exist; this antibody detects both. |
| Entrez Gene | 23028 |
|---|---|
| Protein Accession | NP_001009999 |
Application
Immunohistochemistry of LSD1 in human spleen tissue with LSD1 antibody at 5 μg/mL.
Application
Western blot analysis of LSD1 in A549 cell lysate with LSD1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | LSD1 antibody can be used for detection of LSD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LSD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | LSD1 antibody is supplied in PBS containing 0.02% sodium azide. |
