Application
Flow cytometric analysis of HepG2 cells using LTA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of LTA Antibody in MDA-MB231 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | LTA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, TNFB, TNFSF1 |
| Product description | LTA, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.1) Buonaguro,L., et.al., J. Virol. 66 (12), 7159-7167 (1992) |
| Immunogen | This LTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the Central region of human LTA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LTA |
|---|---|
| Alternative Names | Lymphotoxin-alpha LT-alpha TNF-beta Tumor necrosis factor ligand superfamily member 1 LTA TNFB TNFSF1 |
| Molecular Weight(MW) | 22 kDa |
Application
Flow cytometric analysis of HepG2 cells using LTA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of LTA Antibody in MDA-MB231 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
