Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of LUM antibody in A375 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with LUM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | LUM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican, LUM, LDC, SLRR2D |
| Product description | LUM is a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, this protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. The protein is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.1) Wang,P., Li,S. Invest. Ophthalmol. Vis. Sci. 50 (4), 1546-1551 (2009) |
| Immunogen | This LUM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the Central region of human LUM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LUM |
|---|---|
| Alternative Names | Lumican Keratan sulfate proteoglycan lumican KSPG lumican LUM LDC SLRR2D |
| Molecular Weight(MW) | 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of LUM antibody in A375 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with LUM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
