Application
M-CSF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of M-CSF Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody at 1:1000 at each lane.Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | M-CSF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Processed macrophage colony-stimulating factor 1, CSF1 |
| Product description | CSF1 is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. This protein may be involved in development of the placenta.1) Lee,M.S., et.al.,J. Immunol. 183 (5), 3390-3399 (2009) |
| Immunogen | This M-CSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-257 amino acids from the Central region of human M-CSF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CSF1 |
|---|---|
| Alternative Names | Macrophage colony-stimulating factor 1 CSF-1 M-CSF MCSF Lanimostim Processed macrophage colony-stimulating factor 1 CSF1 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
M-CSF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of M-CSF Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody at 1:1000 at each lane.
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
