Application
Western blot analysis of MAGEA9 using rabbit polyclonal MAGEA9 Antibody (C185) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.Application
Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MAGEA9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAGEA9 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.1) Rogner, U.C., et al., Genomics 29(3):725-731 (1995). |
| Immunogen | This MAGEA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human MAGEA9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MAGEA9 |
|---|---|
| Alternative Names | Melanoma-associated antigen 9 Cancer/testis antigen 19 CT19 MAGE-9 antigen MAGEA9 MAGE9 MAGEA9A |
| Molecular Weight(MW) | 35 kDa |
| Entrez Gene | 4108, 728269 |
|---|---|
| Protein Accession | P43362 |
Application
Western blot analysis of MAGEA9 using rabbit polyclonal MAGEA9 Antibody (C185) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.
Application
Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
