Application
Immunohistochemistry staining of MALT1 in colon, lymphoid follicle tissue using MALT1 Antibody.| Product Name | MALT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MALT1, Caspase-like protein, MLT, MLT1, Paracaspase, MALT associated translocation |
| Product description | MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain. It binds to Bcl10 through its Ig-like domains and cooperates with Bcl10 to activate NF-kappaB. MALT1 is thought to play an importantrole in NF-kappaB signaling by enchancing NF-kappaB activation through interaction with Bcl10 (Ho et al., 2005). Interaction between MALT1 and Bcl10 mediates IKK activation in vitro through facilitatiing the ubiquitination of NEMO by the ubiquitin-conjugating enzyme UBC13. Four recurrent chromosomal translocations have been described in non-Hodgkin B-cell lymphoma of the mucosa-associated lymphoid issue (MALT) type. Two of them,t(14;18)(q32;q21) and t(11;18)(q21;q21), are found in 30% to 50% of extranodal MALT lymphomas. The first translocation juxtaposes the MALT1 gene to the IgH promoter region, resulting in the deregulation of MALT1 expression, whereas the second translocation generates a fusion between MALT1 and the inhibitor of apoptosis gene API2 (cIAP2/HIAP1). The other two much rarer translocations, t(1;14)(p22;q32)and t(1;2)(p22;p12), target Bcl10 gene on the short arm of chromosome 1. Translocations involving Bcl10 and MALT1 may lead to elevated NF-kappaB activity in MALT B-cell lymphoma. Two alternatively spliced transcript variants encoding different isoforms (a and b) have been described for the MALT1 gene. Human MALT1 isoform a is an amino acid protein of 824 amino acids, and human MALT1 isoform b is an amino acid protein of 813 amino acids. |
| Immunogen | A synthetic peptide corresponding to amino acids 43-57 (ELLDQAPEGRGWRRL) of human MALT1 (GenBank No. NP_776216.1). The immunogen sequence is 100% conserved between Malt1 is oforms a and b. Percent identity by BLAST analysis: Human (100%); Monkey (93%) ... |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| MALT1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | MALT1 |
|---|---|
| Alternative Names | MALT1 Caspase-like protein MLT MLT1 Paracaspase MALT associated translocation |
Application
Immunohistochemistry staining of MALT1 in colon, lymphoid follicle tissue using MALT1 Antibody.| Application Notes | MALT1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MALT1 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
