Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of MAP2K5 using rabbit polyclonal MAP2K5 Antibody (S149).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2).| Product Name | MAP2K5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5 |
| Product description | MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.1) Raviv,Z.,J. Cell. Sci. 117 (PT 9), 1773-1784 (2004) |
| Immunogen | This MAP2K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from human MAP2K5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K5 |
|---|---|
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 5 MAP kinase kinase 5 MAPKK 5 MAPK/ERK kinase 5 MEK 5 MAP2K5 MEK5 MKK5 PRKMK5 |
| Molecular Weight(MW) | 50 kDa |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of MAP2K5 using rabbit polyclonal MAP2K5 Antibody (S149).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
