Application
Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | MAP2K7 (phospho Ser271) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKMK7, JNKK2, MKK7, MAP2K7, MP2K7 |
| Product description | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.1) Wu Z., Mol. Cell. Biol. 17:7407-7416(1997). |
| Immunogen | MAP2K7 (phospho Ser271) antibody was raised against a peptide sequence around phosphorylation site of Serine271 (V-D-S (p)-K-A) derived from Human MAP2K7. |
| Modification | p-Ser271 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K7 |
|---|---|
| Alternative Names | PRKMK7 JNKK2 MKK7 MAP2K7 MP2K7 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MAP2K7 only when phosphorylated at serine 271. |
| Entrez Gene | 5609 |
|---|---|
| Protein Accession | NP_660186.1 |
Application
Immunohistochemical analysis of paraffin-embedded humanbrain tissue, using MAP2K7 (Phospho-Ser271) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells treated with insulin using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
